In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface. It is a commonly used laboratory test to detect antibodies in the blood Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid
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Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy.
What does elisa detect and measure
An elisa is an indirect measure of the binding of antibody with antigen The quality of the assay will depend in large part on the specificity of the antibody for the antigen If the specificity is poor, there will be a highly nonspecific background. Elisa tests can detect hormones, bacterial antigens, and antibodies
Read on to learn how the elisa test works and how it's used.