It's widely used to treat chest infections such as pneumonia, infections of the nose and throat such as sinus infection (sinusitis), skin infections, lyme disease, and some sexually transmitted infections. One such tool, zeocin, has garnered attention in mammalian cell culture Zeocin is defined as a glycopeptide antibiotic and a formulation of phleomycin d1, belonging to the bleomycin family, known for its ability to generate hydroxyl radicals and cleave dna
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It is utilized as a selection agent in cell line development, although it can cause mutagenic damage despite the presence of resistance genes
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Overview zeocin™ is a member of the bleomycin/phleomycin family of antibiotics isolated from streptomyces It shows strong toxicity against bacteria, fungi (including yeast), plants and mammalian cell lines (calmels et al., 1991 Since zeocin™ is active in both bacteria and mammalian cell lines. Zeocin® is a member of bleomycin family and acts by intercalating dna causing dna to cleavage and resulting in cell death
Sh ble gene codes a 13665 dalton protein in order for the cell to be zeocin® resistant This protein can be synthesized on the antibiotic which prevents itself from binding to dna and therefore, has resistance to zeocin®. Intro the landscape of cell biology has transformed vastly in recent years, offering tools and methodologies that push the boundaries of research and therapeutic applications
