Methods for determining bactericidal activity of antimicrobial agents To do this, we used the minimum bactericidal concentration (mbc) determination approach to define bactericidal or bacteriostatic activity. Approved guideline this document provides procedures for determining the lethal activity of antimicrobial agents.
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This clsi guideline provides standardized laboratory methods for assessing bactericidal activity, ensuring accurate and reproducible testing for antimicrobial agents.
The minimum bactericidal concentration (mbc) test can be used to assess the ability of an antimicrobial agent to kill a bacterial isolate
Mbc tests are performed after consultation in very select clinical situations when it is necessary to determine the bactericidal activity of an antimicrobial agent against a bacterial isolate. The mbc is determined using a series of steps, undertaken after a minimum inhibitory concentration (mic) test has been completed Also termed minimum lethal concentration (mlc), the mbc of an antibacterial agent is determined by subculturing the last clear mic tube onto the growth medium and examining for bacterial growth. This study aimed to optimize the treatment of ntm infection by selecting antibiotics with bactericidal activity for combination therapy
