Here, the authors implement multispectral fluorescence lifetime imaging microscopy. Unlike the fluorescence intensity, the fluorescence lifetime generally does not depend on the concentration of the fluorophore. Fluorescence lifetimes were spatially mapped on graphite electrodes during lithiation and delithiation within two electrolyte formulations containing either a li [pf 6] or li [clo 4] salt, which highlighted differing emission characteristics associated with salt.
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Fluorescence lifetime imaging (flim) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes
It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because flim is largely independent of the local fluorophore concentration and excitation intensity
Many flim applications relevant for. Fluorescence lifetime imaging microscopy (flim) is a method for measuring fluorophore lifetimes with microscopic spatial resolution, providing a useful tool for cell biologists to detect, visualize, and investigate structure and function of biological systems In this chapter, we begin by introducing the basic theory of fluorescence lifetime, including the characteristics of fluorophore decay.
